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Practical Manual of Experimental and Clinical Pharmacology Practical Manual of Experimental and Clinical Pharmacology Bikash Medhi MBBS, MD(AIIMS), MAMS, FIMSA Associate Professor Department of Pharmacology Research Block B, 4th Floor Postgraduate Institute of Medical Education and Research Chandigarh, 160012, India E-mail: drbikashus@yahoo.com Ajay Prakash MSc (Pharmacology), DPPM, DPMM Ex. Jr. Demonstrator Department of Pharmacology Research Block B, 4th Floor Postgraduate Institute of Medical Education and Research Chandigarh, 160012, India E-mail: ajayprakashpgi@gmail.com ® JAYPEE BROTHERS MEDICAL PUBLISHERS (P) LTD New Delhi • St Louis (USA) • Panama City (Panama) • London (UK) • Ahmedabad Bengaluru • Chennai • Hyderabad • Kochi • Kolkata • Lucknow • Mumbai • Nagpur Published by Jitendar P Vij Jaypee Brothers Medical Publishers (P) Ltd Corporate Office 4838/24 Ansari Road, Daryaganj, New Delhi - 110002, India, Phone: +91-11-43574357, Fax: +91-11-43574314 Registered Office B-3 EMCA House, 23/23B Ansari Road, Daryaganj, New Delhi - 110 002, India Phones: +91-11-23272143, +91-11-23272703, +91-11-23282021 +91-11-23245672, Rel: +91-11-32558559, Fax: +91-11-23276490, +91-11-23245683 e-mail: jaypee@jaypeebrothers.com, Website: www.jaypeebrothers.com Offices in India • Ahmedabad, Phone: Rel: +91-79-32988717, e-mail: ahmedabad@jaypeebrothers.com • Bengaluru, Phone: Rel: +91-80-32714073, e-mail: bangalore@jaypeebrothers.com • Chennai, Phone: Rel: +91-44-32972089, e-mail: chennai@jaypeebrothers.com • Hyderabad, Phone: Rel:+91-40-32940929, e-mail: hyderabad@jaypeebrothers.com • Kochi, Phone: +91-484-2395740, e-mail: kochi@jaypeebrothers.com • Kolkata, Phone: +91-33-22276415, e-mail: kolkata@jaypeebrothers.com • Lucknow, Phone: +91-522-3040554, e-mail: lucknow@jaypeebrothers.com • Mumbai, Phone: Rel: +91-22-32926896, e-mail: mumbai@jaypeebrothers.com • Nagpur, Phone: Rel: +91-712-3245220, e-mail: nagpur@jaypeebrothers.com Overseas Offices • North America Office, USA, Ph: 001-636-6279734, e-mail: jaypee@jaypeebrothers.com, anjulav@jaypeebrothers.com • Central America Office, Panama City, Panama, Ph: 001-507-317-0160, e-mail: cservice@jphmedical.com Website: www.jphmedical.com • Europe Office, UK, Ph: +44 (0) 2031708910, e-mail: dholman@jpmedical.biz Practical Manual of Experimental and Clinical Pharmacology © 2010, Jaypee Brothers Medical Publishers (P) Ltd. The authors of this book have used their best scientific knowledge and skills to provide correct information of their best capacity, to provide accurate and authentic information with recent update as far as possible based on published data available at national and international level. However, readers are requested to take their own decision in case of any differences of opinion based on published data. All rights reserved. No part of this publication should be reproduced, stored in a retrieval system, or transmitted in any form or by any means: electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of the authors and the publisher. This book has been published in good faith that the material provided by authors is original. Every effort is made to ensure accuracy of material, but the publisher, printer and authors will not be held responsible for any inadvertent error (s). In case of any dispute, all legal matters are to be settled under Delhi jurisdiction only. First Edition: 2010 ISBN 978-81-8448-953-8 Typeset at JPBMP typesetting unit Printed at Ajanta Offset Dedicated to My parents, wife (Dr Sujata Upadhyay), son (Debayan Medhi) and all my students for their constant encouragement during my teaching career Bikash Medhi Dedicated to Mentor “Dr Bikash Medhi” who always encouraged and motivated me to do well My grandma and parents for their emotional caring support and entire family for their constant support whenever I needed To all “True Friends” who are always with me and encouraged me to excel in life Ajay Prakash PREFACE The purpose of the present book is to provide fundamental knowledge of practical aspects of the subject ranging from laboratory animals to clinical aspects and practical implications of v

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